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專家信息：

郭占云，男，1972年出生，河北灤南人，博士�，F任同濟大學生命科學與技術學院蛋白質研究所研究員，博士生導師。

1996年畢業于吉林大學分子生物學系，獲學士學位。同年免試進入中國科學院上海生物化學研究所攻讀博士學位，在馮佑民研究員實驗室從事胰島素超家族多肽研究，2001年8月獲生物化學與分子生物學博士學位。2001-2003年在中國科學院生物化學與細胞生物學研究所工作，歷任助理研究員和副研究員。2003年9月到美國Dartmouth College醫學院從事博士后研究，主要研究膽固醇酯合酶1（ACAT1）的結構與功能。2006年底回國到同濟大學生命科學與技術學院蛋白質研究所工作,主要從事蛋白質多肽激素與G蛋白偶聯受體（GPCR）相互作用研究。以通訊作者或第一作者發表SCI論文60余篇，累計影響因子超過200，被引用1000余次。已主持國家自然科學基金面上項目6項，國家“重大新藥創制”科技重大專項1項，國家重點基礎研究發展計劃課題1項。

教育及工作經歷：

1992年9月-1996年7月，吉林大學分子生物學系，獲學士學位。

1996年9月-2001年6月，中國科學院上海生物化學研究所，在馮佑民研究員實驗室從事胰島素超家族多肽研究。獲生物化學與分子生物學博士學位。

2001年7月至2003年9月，中國科學院上海生命科學研究院生物化學與細胞生物學研究所工作，歷任助理研究員和副研究員。

2003年9月-2006年9月，美國Dartmouth College醫學院生物化學系從事博士后研究，主要研究膽固醇酯合酶1（ACAT1）結構與功能。

2006年12月-2007年6月，同濟大學生命科學與技術學院, 副教授。

2007年6月-至今，同濟大學生命科學與技術學院, 研究員，課題組長。

學術兼職：

1、中國生物化學與分子生物學會理事。

2、上海市生物化學與分子生物學學會理事。

科學研究：

研究方向：

1.多肽激素與GPCR相互作用的機制研究及新型激動劑、拮抗劑的設計開發；

2.從G蛋白偶聯受體中篩選、鑒定孤兒多肽的受體；

3.多肽激素與受體相互作用研究新方法的開發。

承擔科研項目情況：

主持國家自然科學基金面上項目2項；國家“重大新藥創制”科技重大專項（候選藥物）1項；國家重點基礎研究發展計劃（973）課題1項。

1．國家自然科學基金項目：受體結合誘導的HIV外膜糖蛋白gp120二硫鍵的還原及其催化分子。

2．國家自然科學基金項目：尋找類胰島素肽6（INSL6）的受體。

3．國家重點基礎研究發展計劃（973計劃）項目：心肌中局部內源性H2S生成調控及傳遞。

科研成果：

1．胰島素原/胰島素的體外折疊和二硫鍵形成途徑研究馮佑民; 喬志松; 郭占云; 閆菡; 賈曉淵【科技成果】中國科學院上海生命科學研究院生物化學與細胞生物學研究所 2003-01-01

2．胰島素和類胰島素生長因子-1不同折疊行為的分子基礎研究馮佑民; 郭占云; 沈璐; 王帥【科技成果】中國科學院上海生命科學研究院生物化學與細胞生物學研究所 2003-01-15

3．胰島素分子的受體結合部位和胰島素二硫鍵功能的深入研究馮佑民; 郭占云; 唐月華; 陳暉; 閆菡; 賈曉淵【科技成果】中國科學院上海生命科學研究院生物化學與細胞生物學研究所 2002-12-15

4．胰島素及其超家族的結構功能及折疊的研究馮佑民; 唐月華; 郭占云; 陳暉; 喬志松【科技成果】中國科學院上海生命科學研究院生物化學與細胞生物學研究所 2001-12-15

發明專利：

[1]施佳萍,郭占云,何勝祥. 一種重組表達白血病抑制因子的方法[P]. CN103981205A,2014-08-13.

[2]郭占云,王曉敏,何勝祥. 一種抗腫瘤靶向復合物及其制備方法和應用[P]. CN103288968A,2013-09-11.

[3]馮佑民,梁鎮和,張友尚,唐月華,王燕,陳暉,史民,王瓊慶,郭占云,劉濱,朱尚權,許英鎬. 重組天然和新型人胰島素及其制備方法[P]. CN1287124,2001-03-14.

論文專著：

在J Biol Chem, Biochemistry, Protein Science等國內外知名科技期刊上以第一作者（含共同第一作者）或通訊作者（含共同通訊作者）發表SCI論文40余篇。

發表英文論文：

1. Li HZ, Shou LL, Shao XX, Liu YL, Xu ZG, Guo ZY* .Identifying key residues and key interactions for the binding of LEAP2 to receptor GHSR1a. Biochem. J. 2020,477: 3199-3217.

2. Li HZ, Li N, Shao XX, Liu YL, Xu ZG, Guo ZY*. Hydrophobic interactions of relaxin family peptide receptor 3 with ligands identified using a NanoBiT-based binding assay. Biochimie. 2020,177: 117-126.

3. Wang JH, Li HZ, Shao XX, Nie WH, Liu YL, Xu ZG, Guo ZY* (2019) Identifying the binding mechanism of LEAP2 to receptor GHSR1a. FEBS J. 286: 1332-1345.

4. Wang JH, Nie WH, Shao XX, Li HZ, Hu MJ, Liu YL, Xu ZG, Guo ZY* .Exploring electrostatic interactions of relaxin family peptide receptor 3 and 4 with ligands using a NanoBiT-based binding assay. Biochim. Biophys. Acta Biomembr. 2019,1861: 776-786.

5. Wang JH, Shao XX, Hu MJ, Liu YL, Xu ZG, Guo ZY*.Functionality of an absolutely conserved glycine residue in the chimeric relaxin family peptide R3/I5. Amino Acids .2019,51: 619-626.

6. Wang JH, Hu MJ, Zhang L, Shao XX, Lv CH, Liu YL, Xu ZG, Guo ZY* .Exploring receptor selectivity of the chimeric relaxin family peptide R3/I5 by incorporating unnatural amino acids. Biochimie. 2018,154: 77-85.

7. Hu MJ, Shao XX, Li HZ, Nie WH, Wang JH, Liu YL, Xu ZG, Guo ZY* .Development of a novel ligand binding assay for relaxin family peptide receptor 3 and 4 using NanoLuc complementation. Amino Acids 2018,50: 1111-1119.

8. Wang JH, Hu MJ, Shao XX, Wei D, Liu YL, Xu ZG, Guo ZY* .Cholesterol modulates the binding properties of human relaxin family peptide receptor 3 with its ligands. Arch. Biochem. Biophys. 2018,646: 24-30.

9. Hu MJ, Wang JH, Shao XX, Liu YL, Xu ZG, Guo ZY* .Overexpression of relaxin family peptide receptor 3 in Escherichia coli and characterization of its ligand binding properties. Process Biochem. 2018,69: 131-135.

10.Wang Jia Hui; Shao Xiao Xia; Hu Meng Jun; Wei Dian; Nie Wei Han; Liu Ya Li; Xu Zeng Guang; Guo Zhan Yun*.Rapid preparation of bioluminescent tracers for relaxin family peptides using sortase-catalysed ligation.Amino Acids, 2017, 49(9): 1611-1617.

11.Wei Dian; Hu Meng Jun; Shao Xiao Xia; Wang Jia Hui; Nie Wei Han; Liu Ya Li; Xu Zeng Guang; Guo Zhan Yun*.Development of a selective agonist for relaxin family peptide receptor 3.Scientific Reports, 2017, 7(1): 3230.

12.Wang Jia Hui; Shao Xiao Xia; Hu Meng Jun; Wei Dian; Liu Ya Li; Xu Zeng Guang; Guo Zhan Yun*.A novel BRET-based binding assay for interaction studies of relaxin family peptide receptor 3 with its ligands.Amino Acids, 2017, 49(5): 895-903.

13.Hu Meng Jun; Wei Dian; Shao Xiao Xia; Wang Jia Hui; Liu Ya Li; Xu Zeng Guang; Guo Zhan Yun*.Interaction mechanism of insulin-like peptide 5 with relaxin family peptide receptor 4.Archives of Biochemistry and Biophysics, 2017, 619: 27-34.

14.Hu Meng Jun; Shao Xiao Xia; Wang Jia Hui; Wei Dian; Liu Ya Li; Xu Zeng Guang; Guo Zhan Yun*.Identification of hydrophobic interactions between relaxin-3 and its receptor RXFP3: implication for a conformational change in the B-chain C-terminus during receptor binding.Amino Acids, 2016, 48(9): 2227-2236.

15.Liu Yu; Zhang Lei; Shao Xiao Xia; Hu Meng Jun; Liu Ya Li; Xu Zeng Guang; Guo Zhan Yun*.A negatively charged transmembrane aspartate residue controls activation of the relaxin-3 receptor RXFP3.Archives of Biochemistry and Biophysics, 2016, 604: 113-120.

16.Song Ge; Shao Xiao Xia; Wu Qing Ping; Xu Zeng Guang; Liu Ya Li; Guo Zhan Yun.Novel Bioluminescent Binding Assays for Ligand-Receptor Interaction Studies of the Fibroblast Growth Factor Family.PLos One, 2016, 11(7): e0159263.

17.Hu, Meng-Jun; Shao, Xiao-Xia; Wang, Jia-Hui; Wei, Dian; Guo, Yu-Qi; Liu, Ya-Li; Xu, Zeng-Guang; Guo, Zhan-Yun*.Mechanism for insulin-like peptide 5 distinguishing the homologous relaxin family peptide receptor 3 and 4.Scientific Reports, 2016, 6: 29648.

18.Chen Chen; Wang Jiahong; Cai Ruiping; Yuan Yanmeng; Guo Zhanyun; Grewer Christof; Zhang Zhou*.Identification of a Disulfide Bridge in Sodium-Coupled Neutral Amino Acid Transporter 2(SNAT2) by Chemical Modification.PLos One, 2016, 11(6): e0158319.

19.Liu, Ya-Li; Guo, Zhan-Yun*.Novel bioluminescent binding assays for interaction studies of protein/peptide hormones with their receptors.Amino Acids, 2016, 48(5): 1151-1160.

20.Wu, Qing-Ping; Zhang, Lei; Shao, Xiao-Xia; Wang, Jia-Hui; Gao, Yu; Xu, Zeng-Guang; Liu, Ya-Li*; Guo, Zhan-Yun*.Application of the novel bioluminescent ligand-receptor binding assay to relaxin-RXFP1 system for interaction studies.Amino Acids, 2016, 48(4): 1099-1107.

21.Song Ge; Wu Qing Ping; Xu Ting; Liu Ya Li; Xu Zeng Guang; Zhang Shi Fu; Guo Zhan Yun*.Quick preparation of nanoluciferase-based tracers for novel bioluminescent receptor-binding assays of protein hormones: Using erythropoietin as a model.Journal of Photochemistry and Photobiology B: Biology , 2015, 153: 311-316.

22.Wang Xiaomin; Guo Zhanyun*.Anti-gliomas Effect of Chlorotoxin-Conjugated Onconase at High Dose.Cell Biochemistry and Biophysics, 2015, 73(2): 389-392.

23.Liu Yu; Shao Xiao Xia; Zhang Lei; Song Ge; Liu Ya Li; Xu Zeng Guang; Guo Zhan Yun*.Novel bioluminescent receptor-binding assays for peptide hormones: using ghrelin as a model.Amino Acids, 2015, 47(10): 2237-2243.

24.Ji, Ben-Jun; Song, Ge; Zhang, Zhou; Guo, Zhan-Yun*.Efficient overexpression of human interleukin-6 in Escherichia coli using nanoluciferase as a fusion partner.Process Biochemistry, 2015, 50(10): 1618-1622.

25.Guo, Yu-Qi; Wu, Qing-Ping; Shao, Xiao-Xia; Shen, Ting; Liu, Ya-Li; Xu, Zeng-Guang; Guo, Zhan-Yun*.Secretory overexpression and isotopic labeling of the chimeric relaxin family peptide R3/I5 in Pichia pastoris.Amino Acids, 2015, 47(6): 1117-1125.

26.Liu Yu; Song Ge; Shao Xiao Xia; Liu Ya Li; Guo Zhan Yun*.Quantitative measurement of cell membrane receptor internalization by the nanoluciferase reporter: Using the G protein-coupled receptor RXFP3 as a model.Biochimica et Biophysica Acta-Biomembranes, 2015, 1848(2): 688-694.

27.He, Sheng-Xiang; Song, Ge; Shi, Jia-Ping; Guo, Yu-Qi; Guo, Zhan-Yun*.Nanoluciferase as a novel quantitative protein fusion tag: Application for overexpression and bioluminescent receptor-binding assays of human leukemia inhibitory factor.Biochimie, 2014, 106: 140-148.

28.Wang, Xin-Yi; Guo, Yu-Qi; Shao, Xiao-Xia; Liu, Ya-Li; Xu, Zeng-Guang; Guo, Zhan-Yun*.Identification of important residues of insulin-like peptide 5 and its receptor RXFP4 for ligand-receptor interactions.Archives of Biochemistry and Biophysics, 2014, 558: 127-132.

29.Wang, Xin-Yi; Guo, Yu-Qi; Zhang, Wei-Jie; Shao, Xiao-Xia; Liu, Ya-Li; Xu, Zeng-Guang; Guo, Zhan-Yun*.The electrostatic interactions of relaxin-3 with receptor RXFP4 and the influence of its B-chain C-terminal conformation.FEBS Journal, 2014, 281(13): 2927-2936.

30.Zhang, Wei-Jie; Wang, Xin-Yi; Guo, Yu-Qi; Luo, Xiao; Gao, Xue-Juan; Shao, Xiao-Xia; Liu, Ya-Li; Xu, Zeng-Guang; Guo, Zhan-Yun*.The highly conserved negatively charged Glu141 and Asp145 of the G-protein-coupled receptor RXFP3 interact with the highly conserved positively charged arginine residues of relaxin-3.Amino Acids, 2014, 46(5): 1393-1402.

31.Zhang, Lei; Song, Ge; Xu, Ting; Wu, Qing-Ping; Shao, Xiao-Xia; Liu, Ya-Li; Xu, Zeng-Guang; Guo, Zhan-Yun*.A novel ultrasensitive bioluminescent receptor-binding assay of INSL3 through chemical conjugation with nanoluciferase.Biochimie, 2013, 95(12): 2454-2459.

32.Wang, Xiao-Min; Luo, Xiao; Guo, Zhan-Yun*.Recombinant expression and downstream processing of the disulfide-rich tumor-targeting peptide chlorotoxin.EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2013, 6(4): 1049-1053.

33.Zhang, Wei-Jie; Jiang, Qian; Wang, Xin-Yi; Song, Ge; Shao, Xiao-Xia; Guo, Zhan-Yun*. A convenient method for europium-labeling of a recombinant chimeric relaxin family peptide R3/I5 for receptor-binding assays.Journal of Peptide Science, 2013, 19(6): 350-354.

34.Song, Ge; Jiang, Qian; Xu, Ting; Liu, Ya-Li; Xu, Zeng-Guang; Guo, Zhan-Yun*.A convenient luminescence assay of ferroportin internalization to study its interaction with hepcidin.FEBS Journal, 2013, 280(8): 1773-1781.

35.Zhang, Wei-Jie; Gao, Xue-Juan; Liu, Ya-Li; Shao, Xiao-Xia; Guo, Zhan-Yun*.Design, recombinant preparation and europium-labeling of a fully active easily-labeled INSL3 analog for receptor-binding assays.Process Biochemistry, 2012, 47(12): 1856-1860.

36.Luo, Xiao; Jiang, Qian; Song, Ge; Liu, Ya-Li; Xu, Zeng-Guang; Guo, Zhan-Yun*.Efficient oxidative folding and site-specific labeling of human hepcidin to study its interaction with receptor ferroportin.FEBS Journal, 2012, 279(17): 3166-3175.

37.Zhang, Wei-Jie; Luo, Xiao; Liu, Ya-Li; Shao, Xiao-Xia; Wade, John D.; Bathgate, Ross A. D.; Guo, Zhan-Yun*.Site-specific DOTA/europium-labeling of recombinant human relaxin-3 for receptor-ligand interaction studies.Amino Acids, 2012, 43(2): 983-992.

38.Zhang, Wei-Jie; Luo, Xiao; Song, Ge; Wang, Xin-Yi; Shao, Xiao-Xia; Guo, Zhan-Yun*.Design, recombinant expression and convenient A-chain N-terminal europium-labelling of a fully active human relaxin-3 analogue.FEBS Journal, 2012, 279(8): 1505-1512.

39.Chen, Guo-Wu; Luo, Xiao; Liu, Ya-Li; Jiang, Qian; Qian, Xian-Ming; Guo, Zhan-Yun*.R171H missense mutation of INSL6 in a patient with spermatogenic failure.European Journal of Medical Genetics, 2011, 54(4): e455-e457.

40.Luo, Xiao; Huang, Chun-Hua; Shao, Xiao-Xia; Guo, Zhan-Yun*.Design, recombinant expression and in vitro maturation of human insulin-like peptide 6 and a biotin-labeled analogue.Process Biochemistry, 2011, 46(6): 1243-1247.

41.Luo, Xiao; Liu, Ya-Li; Layfield, Sharon; Shao, Xiao-Xia; Bathgate, Ross A. D.*; Wade, John D.; Guo, Zhan-Yun.A simple approach for the preparation of mature human relaxin-3.Peptides, 2010, 31(11): 2083-2088.

42.Wu, Xuechen; Shao, Xiaoxia; Guo, Zhan-Yun; Chi, Cheng-Wu.Identification of neuropeptide Y-like conopeptides from the venom of Conus betulinus. Acta Biochimica et Biophysica Sinica, 2010, 42(7): 502-505.

43. Luo X, Bathgate RA, Zhang WJ, Liu YL, Shao XX, Wade JD*, Guo ZY*. (2010) Design and recombinant expression of insulin-like peptide 5 (INSL5) precursors and the preparation of mature human INSL5. Amino Acids, 2010, 39(5): 1343-1352. (* corresponding authors)

44. Wu XC, Zhou M, Peng C, Shao XX, Guo ZY*, Chi CW* (2010) Novel conopeptides in a form of disulfide-crosslinked dimer. Peptides, 2010, 31(6): 1001-1006. (* corresponding authors)

45. Wang ZQ, Zhou ZM, Guo ZY*, Chi CW* (2010) Snapshot of the interaction between HIV envelope glycoprotein 120 and protein disulfide isomerase. Acta Biochimica et Biophysica Sinica, 2010, 42(5): 358-362. (* corresponding authors)

46. Zhang WJ, Luo X, Guo ZY* (2010) In Vitro Degradation of Insulin-like Peptide 3 by Insulin-Degrading Enzyme. Protein Journal, 2010, 29(2): 93-98. (* corresponding author)

47. Luo X, Bathgate RA, Liu YL, Shao XX, Wade JD*, Guo ZY*. (2009) Recombinant expression of an insulin-like peptide 3 (INSL3) precursor and its enzymatic conversion to mature human INSL3. FEBS Journal, 2009, 276(18): 5203-5211. (*corresponding authors)

48. Yuan, Duo-Duo; Liu, Li; Shao, Xiao-Xia; Peng, Can; Chi, Cheng-Wu; Guo, Zhan-Yun* (2009) New conotoxins define the novel I3-superfamily. PEPTIDES, 2009, 30(5): 861-865. (*corresponding authors)

49. Liu, Ya-Li; Jiang, Shu; Ke, Zu-Min; Wu, Hai-Shui; Chi, Cheng-Wu; Guo, Zhan-Yun* (2009) Recombinant expression of a chitosanase and its application in chitosan oligosaccharide production. CARBOHYDRATE RESEARCH, 2009, 344(6): 815-819. (*corresponding author)

50.Zhao Jie; Huang Qi Long; Tang Yue Hua; Guo Zhan Yun; Qiao Zhi Song; Xu Gen Jun; Feng You Min*.Equilibrium folding of porcine insulin precursor in the presence of redox buffer: Implications for the common intermediates shared by its unfolding/refolding processes.Protein and Peptide Letters, 2008, 15(9): 972-979.

51. Yuan DD, Liu L, Shao XX, Peng C, Chi CW*, Guo ZY* (2008) Isolation and cloning of a conotoxin with a novel cysteine pattern from Conus caracteristicus. Peptides, 2008, 29(9): 1521-1525. (*corresponding authors)

52. Qian J, Guo ZY*, Chi CW*. (2008) Cloning and isolation of a conus cysteine-rich protein homologous to Tex31 but without proteolytic activity. Acta Biochimica et Biophysica Sinica, 2008, 40(2): 174-181. (*corresponding authors)

53. Zhang ZJ, Wu L, Qiao ZS, Qiao MQ, Feng YM*, Guo ZY* (2008) Contribution of the conserved A16Leu to insulin foldability. Protein Journal, 2008, 27(3): 192-196. (*corresponding authors)

54. Guo ZY*, Qiao ZS*, Feng YM (2008) The in vitro oxidative folding of the insulin superfamily. Antioxidants and Redox Signaling, 2008, 10(1): 127-139. (*co-first authors)

55. Guo ZY, Chang CCY, Chang TY (2007) Functionality of the seventh and eighth transmembrane domains of acyl-coenzyme A:cholesterol acyltransferase 1. Biochemistry,46: 10063-10071.

56. Wang, Zhi-Qiang; Han, Yu-Hong; Shao, Xiao-Xia; Chi, Cheng-Wu; Guo, Zhan-Yun* (2007) Molecular cloning, expression, and characterization of protein disulfide isomerase from Conus marmoreus. FEBS JOURNAL, 2007, 274(18): 4778-4787..(*corresponding authors)

57. Guo ZY, Lin S, Henien JA, Chang CY, Chang TY (2005) The active site His-460 of human acyl-coenzyme A: cholesterol acyltransferase 1 resides in a hitherto undisclosed transmembrane domain. J Biol Chem, 280: 37814-37826.

58. Guo ZY, Chang CY, Lu X, Chen J, Li BL, Chang TY (2005) The disulfide linkage and the free sulfhydryl accessibility of acyl-coenzyme A:cholesterol acyltransferase 1 as studied by using mPEG5000-maleimide. Biochemistry, 44: 6537-6546.

59. Guo ZY, Zhang Z, Jia XY, Tang YH, Feng YM (2005) Mutational analysis of the absolutely conserved B8Gly: consequence on foldability and activity of insulin. Acta Biochim Biophys Sin,37: 673-679.

60. Guo ZY, Jia XY, Feng YM (2004) Replacement of the interchain disulfide-forming amino acids A7 and B7 by glutamate impairs the structure and activity of insulin. Biol Chem, 385:1171-1175.

61. Guo ZY, Wang S, Tang YH, Feng YM (2004) Mutagenesis of the three conserved valine residues: consequence on the foldability of insulin. Biochim Biophys Acta, 1699: 103-109.

62. Jia XY*, Guo ZY*, Wang Y, Xu Y, Duan SS, Feng YM (2003) Peptide models of four possible insulin folding intermediates with two disulfides. Protein Sci, 12: 2412-2419. (*co- first authors)

63. Wang S*, Guo ZY*, Shen L, Feng YM (2003) Refolding of the amphioxus insulin-like peptide: implications of a bifurcating evolution of the different folding behaviors of insulin and insulin-like growth factor 1. Biochemistry, 42: 9687-9693. (*co-first authors)

64. Guo ZY, Tang YH, Wang S, Feng YM (2003) Contribution of the absolutely conserved B8Gly to the foldability of insulin. Biol Chem, 384: 805-809.

65. Yan H*, Guo ZY*, Gong YW, Xi D, Feng YM (2003) A peptide model of insulin folding intermediate with one disulfide. Protein Sci, 12: 768-775. (*co-first authors)

66. Guo ZY, Shen L, Gu W, Wu AZ, Ma JG., Feng YM (2002) In vitro evolution of amphioxus insulin-like peptide to mammalian insulin. Biochemistry, 41: 10603-10607.

67. Guo ZY, Shen L, Feng YM (2002) The different energetic state of the intra A-chain/ domain disulfide of insulin and insulin-like growth factor 1 is mainly controlled by their B-chain/domain. Biochemistry, 41: 10585-10592.

68. Guo ZY, Shen L, Feng YM (2002) The different folding behavior of insulin and insulin-like growth factor 1 is mainly controlled by their B-chain/domain. Biochemistry, 41: 1556-1567.

69. Guo ZY, Feng YM (2002) The thermodynamic stability of insulin disulfides is not affected by the C-domain of insulin-like growth factor 1. Sci China (Series C), 45: 245-250.

70. Guo ZY, Feng YM (2001) Effects of cysteine to serine substitutions in the two inter-chain disulfide bonds of insulin. Biol Chem, 382: 443-448

71. Guo ZY, Tang YH, Zhang Z, Feng YM (2001) Mutational analysis of the three conserved valine residues of insulin and a proposal of “isosteric residue”. IUBMB Life, 52: 309-314.

72. Guo ZY, Qiao ZS, Feng YM (2001) Unfolding of recombinant single-chain insulin in denaturants containing thiol reagents. Acta Biochim Biophys Sin, 33: 65-70.

73. Shen L*, Guo ZY*, Chen Y, Liu LY, Feng YM (2001) Expression, purification, characterization of ampioxus insulin-like peptide and preparation of polyclonal antibody to it.Acta Biochim Biophys Sin, 33: 629-633. (*co-first authors)

發表中文期刊論文：

[1]黃碧君,黃艷,胡紅琴,郭占云.毛細管電泳法測定重組人源化IgG2抗體的純度[J].中國生物制品學雜志,2018,31(03):287-293+298.

[2]劉亞麗,郭占云.蛋白質多肽激素生物發光配基-受體結合測定新方法[J].生命的化學,2015,35(06):817-822.

[3]邵曉霞,汪世龍,郭占云.蛋白質化學實驗課教學探索[J].課程教育研究,2015(13):147.

[4]周宛虹,邵曉霞,郭占云.吸煙者血清中尼古丁及其多種代謝物的測定與代謝分析[J].現代科學儀器,2013(01):122-125.

[5]田昀,張柿平,郭占云.酶解法制備殼寡糖的初步研究[J].安徽農業科學,2010,38(04):1684-1686.

[6]王志強,周智敏,郭占云.蛋白質二硫鍵異構酶家族的結構與功能[J].生命科學研究,2009,13(06):548-553.

[7]徐瑞,郭占云,戚正武.殼聚糖酶的純化、基因克隆、及其酶學特性的鑒定[J].藥物生物技術,2008(02):94-99.

[8]何勝祥,陳平,郭占云,邵曉霞.蛋白質組學在神經退行性疾病中的研究進展[J].細胞生物學雜志,2007(02):173-178.

[9]閆菡 ,郭占云 ,馮佑民.大分子擁擠及其對蛋白質折疊的影響[J].陜西醫學雜志,2003(03):246-247.

[10]郭占云,馮佑民.類胰島素生長因子-1的C結構域不影響胰島素二硫鍵的熱力學穩定性[J].中國科學(C輯:生命科學),2002(01):84-88.

[11]沈璐,郭占云,陳衍,劉蘭英,馮佑民.文昌魚胰島素樣肽的表達、純化、鑒定及其多克隆抗體制備[J].生物化學與生物物理學報,2001(06):629-633.

[12]郭占云,喬志松,馮佑民.重組單鏈胰島素在含有巰基試劑的變性劑中的解折疊[J].生物化學與生物物理學報,2001(04):431-436.

發表中文會議論文：

[1]彭燦;陳偉華;Tanya Sanders;Geoffrey Chew;Edward Hawrot;戚正武;郭占云. Characterization of two α4/7-conotoxins,Mrl.1 and Lpl.4,as novel antagonists of nicotinic acetylcholine receptors[C]. 第九屆中國生物毒素學術研討會論文摘要. 2009:61.

[2]吳雪塵; 周宓; 郭占云; 戚正武. Novel disulfide-crosslinked dimeric conopeptides cloned based on M-superfamily signal peptide[C]. 第九屆中國生物毒素學術研討會論文摘要. 2009:63.

[3]袁多多;劉麗;郭占云;戚正武. 新型I3-超家族芋螺毒素的分離純化及基因克隆[C]. 第八屆中國生物毒素學術研討會論文摘要集. 2007:102.

[4]袁多多;劉麗;郭占云;戚正武. 從唐草芋螺中分離及克隆含有新型半胱氨酸骨架的芋螺毒素[C]. 第八屆中國生物毒素學術研討會論文摘要集. 2007:105.

[5]袁多多;劉麗;郭占云;戚正武. 唐草芋螺中α-芋螺毒素的分離純化及基因克隆[C]. 第八屆中國生物毒素學術研討會論文摘要集. 2007:106.

[6]袁多多; 劉麗; 郭占云; 戚正武. 新型I3-超家族芋螺毒素的分離純化及基因克隆[C]. 第八屆中國生物毒素學術研討會論文摘要. 2007:102.

[7]袁多多; 劉麗; 郭占云; 戚正武. 從唐草芋螺中分離及克隆含有新型半胱氨酸骨架的芋螺毒素[C]. 第八屆中國生物毒素學術研討會論文摘要. 2007:105.

[8]袁多多; 劉麗; 郭占云; 戚正武. 唐草芋螺中α-芋螺毒素的分離純化及基因克隆[C 第八屆中國生物毒素學術研討會論文摘要. 2007:106.

媒體報道：

開拓多肽研究新篇章

——記同濟大學生命科學與技術學院蛋白質研究所郭占云研究員

多肽是氨基酸通過肽鍵連接在一起而形成的聚合物，通常分子量小于6000，有些由單獨的基因編碼，有些是蛋白質的水解產物。它們在體內主要作為調控分子發揮作用，例如大家熟知的胰島素、生長激素等。我國在多肽研究上取得過一系列重大成果。在此，同濟大學生命科學與技術學院蛋白質研究所郭占云研究員具有多年的多肽學習和研究經歷，并將帶來一些新成果。

體內合成時，絕大多數多肽在基因的指導下先合成一個前體，然后再經過一系列加工轉變為具有生物活力的成熟形式，例如胰島素就是先在胰島β細胞中合成單鏈的“前胰島素原”，再轉變為“胰島素原”，最后形成兩條肽鏈的“胰島素”。多肽發揮調控功能多是靠激活細胞膜上的特定受體，從而引發細胞內一系列信號傳遞，最終使細胞對外界環境做出反應。例如胰島素就是通過激活細胞膜上的胰島素受體使細胞對高血糖水平做出反應，即把血液中的葡萄糖運輸到細胞內從而使血糖濃度維持在正常水平。如果人體內不能合成足量的胰島素（1型糖尿病就屬于這種情況），體內的血糖水平就會升高，過多的葡萄糖就會通過尿液排出體外，產生糖尿病。要治療這種1型糖尿病，就需要注射胰島素以補充內源胰島素的不足�，F在使用的胰島素，都是通過基因過程的方法利用微生物生產的，但是微生物合成后還要經過一系列處理才能生產出有活力的藥用胰島素。

郭老師于1996年從吉林大學分子生物學系畢業，后進入上海生物化學研究所馮佑民研究員實驗室，開始從事胰島素超家族的結構、功能及折疊等研究工作。2003年到美國做博士后，所從事的膜蛋白研究為后來研究多肽與受體的相互作用打下了基礎。

2006年，郭老師回國到同濟大學蛋白質研究所工作，開始逐步建立起了自己的實驗室，又開始了多肽的相關研究工作，除胰島素外，人基因組還編碼9個與胰島素氨基酸序列類似但生理功能不同的活性多肽，可統稱為“類胰島素肽”。郭老師實驗室通過大腸桿菌重組表達與體外成熟相結合的策略，解決了這些二硫鍵交聯的雙鏈類胰島素肽的制備，并進一步通過定點鑭系金屬離子標記的方法獲得了標記的類胰島素肽，建立了高通量非放射性的受體結合測定體系。現在正利用各種類胰島素肽類似物及受體類似物，通過受體結合與受體激活測定，研究這些同源多肽如何區分、結合及激活它們的受體。此外，最近他們還在體內鐵代謝調控激素Hepcidin與鐵外運蛋白Fpn相互作用方面開展了一些工作，以期了解它們相互作用的細節。

科研路漫漫，只怕有心人，也讓我們期待郭占云老師在這一多肽研究領域摘取更多收獲！

文章來源：《科學中國人》2012-10-24